nūhou

ʻO ka hoʻohālikelike ʻana i nā kūlana physiological e kōkua i nā mea noiʻi e ʻimi i nā mea hoʻopaʻa metala

Ua hoʻomohala nā mea noiʻi i kahi ʻano no ka ʻike ʻana i nā molekole liʻiliʻi e hoʻopaʻa i nā ion metala. He mea nui nā iona metala i ka biology. Akā ʻo ka hoʻomaopopo ʻana i nā molekala-a ʻoi aku hoʻi i nā molekole liʻiliʻi-ʻo ia mau iona metala hiki ke paʻakikī.

No ka hoʻokaʻawale ʻana i nā metabolites no ka nānā ʻana, hoʻohana nā ʻano metabolomic maʻamau i nā solvents organik a me nā pH haʻahaʻa, hiki ke hoʻokaʻawale i nā paʻakikī metala. Ua makemake ʻo Pieter C. Dorrestein o ke Kulanui o Kaleponi San Diego a me nā hoa hana e hoʻopaʻa i nā paʻakikī no ka nānā ʻana ma o ka hoʻohālike ʻana i nā kūlana maoli i loaʻa i loko o nā cell. Akā inā hoʻohana lākou i nā kūlana physiological i ka wā o ka hoʻokaʻawale ʻana o nā molekala, pono lākou e hoʻoponopono hou i nā kūlana kaʻawale no kēlā me kēia kūlana physiological a lākou i makemake ai e hoʻāʻo.

Akā, ua hoʻomohala nā mea noiʻi i kahi ala ʻelua e hoʻolauna i nā kūlana physiological ma waena o ka hoʻokaʻawale chromatographic maʻamau a me kahi loiloi spectrometric mass (Nat. Chem. 2021, DOI: 10.1038/s41557-021-00803-1). ʻO ka mea mua, ua hoʻokaʻawale lākou i kahi unuhi olaola me ka hoʻohana ʻana i ka chromatography wai hana kiʻekiʻe. A laila hoʻoponopono lākou i ka pH o ke kahe e puka ana i ke kolamu chromatographic e hoʻohālike i nā kūlana physiological, hoʻohui i nā ion metala, a nānā i ka hui ʻana me ka spectrometry lehulehu. Ua holo ʻelua lākou i ka loiloi no ka loaʻa ʻana o ka nui o nā molekole liʻiliʻi me nā metala ʻole. No ka ʻike ʻana i nā molekala e hoʻopaʻa ai i nā metala, ua hoʻohana lākou i ke ʻano helu helu e hoʻohana ana i nā kiʻi kiʻekiʻe e hoʻomaopopo i nā pilina ma waena o ke kiko o nā mana paʻa a paʻa ʻole.

ʻO kahi ala e hoʻohālikelike ai i nā kūlana physiological, wahi a Dorrestein, ʻo ia ka hoʻohui ʻana i nā kiʻekiʻe kiʻekiʻe o nā ion e like me ka sodium a i ʻole potassium a me nā haʻahaʻa haʻahaʻa o ka metala hoihoi. “E lilo ia i hoʻokolohua hoʻokūkū. E haʻi maoli ʻo ia iā ʻoe, OK, ʻoi aku ka makemake o kēia mole ma lalo o kēlā mau kūlana e hoʻopaʻa i ka sodium a me ka potassium a i ʻole kēia metala kūikawā āu i hoʻohui ai, "wahi a Dorrestein. "Hiki iā mākou ke hoʻokomo i nā metala like ʻole i ka manawa like, a hiki iā mākou ke hoʻomaopopo maoli i ka makemake a me ke koho ʻana i kēlā ʻano."

I loko o nā moʻomeheu moʻomeheu mai Escherichia coli, ua ʻike nā mea noiʻi i nā pūhui paʻa hao i ʻike ʻia e like me yersiniabactin a me aerobactin. I ka hihia o yersiniabactin, ua ʻike lākou hiki iā ia ke hoʻopaʻa i ka zinc.

Ua ʻike ka poʻe noiʻi i nā pūhui hoʻopaʻa metala i loko o nā laʻana e like me ka paʻakikī e like me ka mea i hoʻoheheʻe ʻia mai ke kai. "ʻO ia kekahi o nā laʻana paʻakikī loa aʻu i nānā ai," wahi a Dorrestein. "He paʻakikī paha ia e like me, inā ʻaʻole i ʻoi aku ka paʻakikī ma mua o ka ʻaila ʻaila." Ua ʻike ʻia ke ʻano o ka ʻakika domoic ma ke ʻano he molekele paʻa keleawe a manaʻo ʻia e hoʻopaʻa iā Cu2+ ma ke ʻano he dimer.

"ʻO kahi ala omics e ʻike ai i nā metabolites paʻa metala āpau i loko o kahi laʻana he mea maikaʻi loa ia ma muli o ke koʻikoʻi o ka chelation metala biological," kākau ʻo Oliver Baars, nāna i aʻo i nā metabolites paʻa metala i hana ʻia e nā mea kanu a me nā microbes ma North Carolina State University, kākau i kahi leka uila.

"Hāʻawi ʻo Dorrestein a me nā hoa hana i kahi hōʻike nani, makemake nui ʻia, e ʻimi maikaʻi ai i ke ʻano o ka hana physiological o nā ion metala i loko o ke kelepona," kākau ʻo Albert JR Heck, he paionia i ka loiloi kikoʻī kikoʻī maoli ma ke Kulanui ʻo Utrecht, kākau ma ka leka uila. "ʻO kahi hana hou aʻe, ʻo ia ka unuhi ʻana i nā metabolites ma lalo o nā kūlana maoli mai ka cell a hoʻokaʻawale i kēia mau mea ma lalo o nā kūlana maoli, e ʻike i nā metabolites e lawe nei i nā ion metala cellular endogenous."

Nūhou Kemika & ʻEnekinia
ISSN 0009-2347
Kuleana kope © 2021 American Chemical Society